Human Alcohol Dehydrogenase 4 (ADH4) ELISA Kit

SKU: orb1291373

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Description

Human ADH4 (Alcohol Dehydrogenase 4) ELISA Kit

Research Area

Metabolism Research, Signal Transduction

Images & Validation

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Item 1 of 1

Tested Applications

ELISA

Application Notes

standard: 50 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human ADH4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ADH4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human ADH4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human ADH4 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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Reactivity	Human
Sample Types	Serum, plasma and other biological fluids.
Assay Type	Sandwich
Assay Time	3.5h
Range	0.79-50 ng/mL
Sensitivity	0.27 ng/mL
Concentration	50 ng/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Disclaimer	For research use only

Alternative Names

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Ac1002, ADH 4, ADH-1, ADH-2, ADH2, Adh4, ADH4_HUMAN, Alcohol dehydrogenase 4 (class II) pi polypeptide, Alcohol dehydrogenase 4, Alcohol dehydrogenase class II pi chain, Alcohol dehydrogenase pi isozyme ADH class II, Aldehyde reductase, Class II alcohol dehydrogenase 4 pi subunit,

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Human Alcohol Dehydrogenase 4 (ADH4) ELISA Kit

Quick Database Links

UniProt

P08319

UniProt Details

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No UniProt data available

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Manual

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Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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