Human Aldose Reductase (AR) ELISA Kit

SKU: orb3146362

Description

This Human Aldose Reductase (AR) ELISA Kit employs a two-site sandwich ELISA to quantitate AKR1B1.

Images & Validation

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Tested Applications

ELISA

Application Notes

This Human Aldose Reductase (AR) ELISA Kit employs a two-site sandwich ELISA to quantitate AKR1B1 in samples. An antibody specific for AKR1B1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyAKR1B1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for AKR1B1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of AKR1B1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Key Properties

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Reactivity	Human
Sample Types	Cell culture supernatants#Serum#Plasma#Other biological fluids
Range	Please inquire
Sensitivity	Please inquire

Storage & Handling

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Storage	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Expiration Date	6 months from date of receipt.
Hazard Information	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Disclaimer	For research use only

Alternative Names

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AKR1B1; ADR; ALDR1; ALR2; AR; MGC1804; Lii5-2 CTCL tumor antigen; aldehyde reductase 1; aldo-keto reductase family 1; member B1; aldose reductase; low Km aldose reductase

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Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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