Human Amyloid Precursor Protein (APP) ELISA Kit

SKU: orb775599

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Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Amyloid Precursor Protein(APP).

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Tested Applications

ELISA

Application Notes

standard: 5000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human APP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human APP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human APP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human APP in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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Reactivity	Human
Sample Types	Serum, plasma, cerebrospinal fluid and other biological fluids
Assay Type	Sandwich
Assay Time	3.5h
Range	78.13-5000 pg/mL
Sensitivity	30 pg/mL
Concentration	5000 pg/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Disclaimer	For research use only

Alternative Names

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AAA ELISA Kit, ABeta ELISA Kit, ABPP ELISA Kit, AD1 ELISA Kit, APPI ELISA Kit, CVAP ELISA Kit, PN2 ELISA Kit, CTFgamma ELISA Kit, Peptidase Nexin-II Alzheimer Disease ELISA Kit, Peptidase Nexin-II ELISA Kit, Amyloid Beta(A4)Precursor Protein ELISA Kit

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Human Amyloid Precursor Protein (APP) ELISA Kit

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UniProt

P05067

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No UniProt data available

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Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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