Human Carbon Catabolite Repression 4 Like Protein (CCRN4L) ELISA Kit

SKU: orb780450

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Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Carbon Catabolite Repression 4 Like Protein(CCRN4L).

Images & Validation

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Tested Applications

ELISA

Application Notes

standard: 20 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CCRN4L. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CCRN4L. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CCRN4L, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CCRN4L in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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Reactivity	Human
Sample Types	Tissue homogenates, cell lysates and other biological fluids.
Assay Type	Sandwich
Assay Time	3.5h
Range	0.32-20 ng/mL
Sensitivity	0.115 ng/mL
Concentration	20 ng/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Disclaimer	For research use only

Alternative Names

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CCR4L ELISA Kit, CCR4 ELISA Kit, NOC ELISA Kit, Nocturnin ELISA Kit, Circadian deadenylase NOC ELISA Kit, Carbon catabolite repression 4-like protein ELISA Kit

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Human Carbon Catabolite Repression 4 Like Protein (CCRN4L) ELISA Kit

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UniProt

Q9UK39

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No UniProt data available

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Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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