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Human CD3 epsilon Protein, His Tag

SKU: orb257326

Description

Human CD3 epsilon, His Tag is expressed from human 293 cells (HEK293). It contains AA Asp 23 - Asp 126 (Accession # P07766-1).

Research Area

Disease Biomarkers

Images & Validation

Application Notes
This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 16.9 kDa. Maybe caused by unknown PTMs, the protein migrates as 18-19 kDa and 21-22 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition, and 35-43 kDa under non-reducing (NR) condition (SDS-PAGE).

Key Properties

Expression SystemHEK293
TargetCD3 epsilon
ReactivityHuman
TagC-10×His
Molecular Weight16.9 kDa
Expression RegionAsp 23 - Asp 126
Purity90%
Endotoxins0.01 EU per μg
ConjugationUnconjugated

Storage & Handling

Storage-20°C
Form/AppearancePowder
Buffer/PreservativesPBS, pH 7.4
Expiration Date6 months from date of receipt.
DisclaimerFor research use only

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Human CD3 epsilon Protein, His Tag

SDS-PAGE analysis of Human CD3 epsilon protein

Human CD3 epsilon Protein, His Tag

Immobilized Anti Human CD3 MAb, mouse IgG1 at 2 ug/mL (100 uL/well) can bind Human CD3E, His Tag with a linear range of 0.4-6.25 ng/mL.

UniProt Details

No UniProt data available

NCBI Reference Sequences

Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product
RefSeqP07766

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Protocol Information

Human CD3 epsilon Protein, His Tag (orb257326)

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100 μg
$ 780.00
1 mg
$ 4,630.00
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