Human Complement Factor H Related Protein 1 (CFHR1) ELISA Kit

SKU: orb780977

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Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Complement Factor H Related Protein 1(CFHR1).

Research Area

Hematology

Images & Validation

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Item 1 of 1

Tested Applications

ELISA

Application Notes

standard: 40 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CFHR1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CFHR1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CFHR1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CFHR1 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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Reactivity	Human
Sample Types	serum, plasma and other biological fluids
Assay Type	Sandwich
Assay Time	3.5h
Range	0.63-40 ng/mL
Sensitivity	0.262 ng/mL
Concentration	40 ng/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Disclaimer	For research use only

Alternative Names

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CFHL ELISA Kit, CFHL1 ELISA Kit, CFHL1P ELISA Kit, CFHR1P ELISA Kit, FHR1 ELISA Kit, H36-1 ELISA Kit, H36-2 ELISA Kit, HFL1 ELISA Kit, HFL2 ELISA Kit, H factor-like protein 1 ELISA Kit

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Human Complement Factor H Related Protein 1 (CFHR1) ELISA Kit

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UniProt

Q03591

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No UniProt data available

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Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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