You have no items in your shopping cart.
Human EGF
SKU: orb3002493
Description
Images & Validation
−
Key Properties
−| Expression System | E.coli |
|---|---|
| Biological Activity | Cell Based Assay: Measured in a cell proliferation assay using BALB/c 3T3 cells. The ED50 for this effect is 60-450 pg/ml (QC verified). Mouse intestinal organoids were cultured with EGF (Cat#orb3002493), Noggin (Cat#orb3002448) and R-spondin 1 (Cat#orb3002497). After five days of culture, the organoids showed good morphology and germination rate. Mouse Colon organoids were cultured with EGF (Cat#orb3002493), Wnt3a (Cat#orb3002512), Noggin (Cat#orb3002448) and R-spondin 1 (Cat#orb3002497). The organoids showed good morphology. Mouse Gastric organoids were cultured with EGF (Cat#orb3002493), Wnt3a (Cat#orb3002512), Noggin (Cat#orb3002448) , R-spondin 1 (Cat#orb3002497) and FGF-10 (Cat#orb3002478). The organoids showed good morphology. Mouse bile duct organoids were cultured with EGF (Cat#orb3002493) , R-spondin 1 (Cat#orb3002497) ,FGF-10 (Cat#orb3002478) and HGF (Cat#orb3002483). The organoids showed good morphology. Human intestinal organoids (ipsc source) were cultured with Activin A(Cat#orb3002480) , BMP-4(Cat#orb3002515) , EGF (Cat#orb3002493) , Wnt3a (Cat#orb3002512), Noggin (Cat#orb3002448), R-spondin 1 (Cat#orb3002497) and FGF-4 (Cat#orb3002445) . The organoids showed good morphology. Human breast cancer organoids were cultured with Wnt3a (Cat#orb3002512), Noggin (Cat#orb3002494) , R-spondin 3 (Cat#orb2993001) , KGF(Cat#orb3002467), FGF-10 (Cat#orb3002478), EGF (Cat#orb3002493) and NRG1-beta 1 (Cat#753). The organoids showed good morphology. |
| Tag | Tag free |
| Molecular Weight | Predicted: 6.2 KDa. Observed: 11 KDa, reducing conditions |
| Expression Region | Asn971-Arg1023 |
| Purity | SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. |
| Endotoxins | < 0.01 EU/µg as determined by LAL test. |
Storage & Handling
−| Storage | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
|---|---|
| Form/Appearance | Lyophilized from a 0.2 μm filtered solution of 20mM Tris,200mM NaCl,4%Mannitol,pH 8.0. |
| Reconstitution | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| Disclaimer | For research use only |
Alternative Names
−Pro-Epidermal Growth Factor; EGF; Epidermal Growth Factor; Urogastrone
Similar Products
−- Item 1 of 1
Human Angiopoietin Receptor Tie1 (ANG-R-Tie1) ELISA Kit [orb1949840]
Human
0.16-10ng/mL
0.1 ng/mL
96 T, 48 T - Item 1 of 1
- Item 1 of 1
Human Milk Fat Globule EGF Factor 8 (MFGE8) ELISA Kit [orb1807354]
Human
62.5-4000pg/mL
25.2 pg/mL
48 T, 96 T - Item 1 of 1
Human Signal Peptide, CUB and EGF-like Domain-containing Protein 1 (SCUBE1) ELISA Kit [orb1947955]
Human
0.63-40ng/mL
0.38 ng/mL
48 T, 96 T - Item 1 of 1
Human Signal Peptide, CUB and EGF-like Domain-containing Protein 3 (SCUBE3) ELISA Kit [orb1947856]
Human
0.79-50ng/mL
0.47 ng/mL
48 T, 96 T
Quality Guarantee
Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at support@biorbyt.com.
Quick Database Links
UniProt
UniProt Details
− No UniProt data available
Documents Download
Datasheet
Product Information
Request a Document
Protocol Information
Human EGF (orb3002493)
- 0.0
Based on 0 reviews
Participating in our Biorbyt product reviews program enables you to support fellow scientists by sharing your firsthand experience with our products.
Login to Submit a ReviewAvailable Sizes
Select a size below
Usually dispatched within 5-10 working days