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Human EGF
SKU: orb3002493
Description
Images & Validation
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Key Properties
−| Expression System | E.coli |
|---|---|
| Biological Activity | Cell Based Assay: Measured in a cell proliferation assay using BALB/c 3T3 cells. The ED50 for this effect is 60-450 pg/ml (QC verified). Mouse intestinal organoids were cultured with EGF (Cat#orb3002493), Noggin (Cat#orb3002448) and R-spondin 1 (Cat#orb3002497). After five days of culture, the organoids showed good morphology and germination rate. Mouse Colon organoids were cultured with EGF (Cat#orb3002493), Wnt3a (Cat#orb3002512), Noggin (Cat#orb3002448) and R-spondin 1 (Cat#orb3002497). The organoids showed good morphology. Mouse Gastric organoids were cultured with EGF (Cat#orb3002493), Wnt3a (Cat#orb3002512), Noggin (Cat#orb3002448) , R-spondin 1 (Cat#orb3002497) and FGF-10 (Cat#orb3002478). The organoids showed good morphology. Mouse bile duct organoids were cultured with EGF (Cat#orb3002493) , R-spondin 1 (Cat#orb3002497) ,FGF-10 (Cat#orb3002478) and HGF (Cat#orb3002483). The organoids showed good morphology. Human intestinal organoids (ipsc source) were cultured with Activin A(Cat#orb3002480) , BMP-4(Cat#orb3002515) , EGF (Cat#orb3002493) , Wnt3a (Cat#orb3002512), Noggin (Cat#orb3002448), R-spondin 1 (Cat#orb3002497) and FGF-4 (Cat#orb3002445) . The organoids showed good morphology. Human breast cancer organoids were cultured with Wnt3a (Cat#orb3002512), Noggin (Cat#orb3002494) , R-spondin 3 (Cat#orb2993001) , KGF(Cat#orb3002467), FGF-10 (Cat#orb3002478), EGF (Cat#orb3002493) and NRG1-beta 1 (Cat#753). The organoids showed good morphology. |
| Tag | Tag free |
| Molecular Weight | Predicted: 6.2 KDa. Observed: 11 KDa, reducing conditions |
| Expression Region | Asn971-Arg1023 |
| Purity | SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. |
| Endotoxins | < 0.01 EU/µg as determined by LAL test. |
Storage & Handling
−| Storage | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
|---|---|
| Form/Appearance | Lyophilized from a 0.2 μm filtered solution of 20mM Tris,200mM NaCl,4%Mannitol,pH 8.0. |
| Reconstitution | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Pro-Epidermal Growth Factor; EGF; Epidermal Growth Factor; Urogastrone
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Human EGF (orb3002493)
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