Human Folic Acid (FA) ELISA Kit

SKU: orb782780

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Description

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Folic Acid(FA) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Folic Acid(FA).

Research Area

Hematology, Metabolism Research, Stem Cell & Developmental Biology

Images & Validation

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Tested Applications

ELISA

Application Notes

standard: 10000 pg/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human FA protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human FA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human FA in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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Reactivity	Human
Sample Types	serum, plasma, cell lysates, cell culture supernates and other biological fluids
Assay Type	Competitive
Assay Time	2h
Range	156.25-10000 pg/mL
Sensitivity	15 pg/mL
Concentration	10000 pg/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Disclaimer	For research use only

Alternative Names

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VB9 ELISA Kit, Vitamin B9 ELISA Kit, Folacin ELISA Kit, Folate ELISA Kit, V ELISA Kit, Itamin M ELISA Kit, Vitamin M ELISA Kit, Vitamin Bc ELISA Kit, Pteroyl-L-Glutamic Acid ELISA Kit, Pteroyl-L-Glutamate ELISA Kit

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Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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