Human IgG F(ab')2 Antibody Peroxidase Conjugated

SKU: orb347292

Description

Human IgG F(ab')2 antibody (Peroxidase)

Images & Validation

−

Tested Applications	ELISA, IHC, WB
Dilution range	ELISA: 1:10,000 - 1:50,000, IHC: 1:500 - 1:2,500, WB: 1:1,000 - 1:10,000
Reactivity	Human
Application Notes	Anti-Human IgG F(ab')2 Antibody has been assayed against 1.0 ug of Human IgG in a standard capture ELISA using ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:20,000 to 1:100,000 of the reconstitution concentration is suggested for Anti-Human IgG F(ab')2 Antibody. Read more...

Key Properties

−

Antibody Type	Secondary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	Human IgG F(ab')2 fragment
Purity	Anti-Human IgG F(ab')2 Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Human IgG, Human IgG F(ab’)2 and Human Serum. No reaction was observed against Human IgG F(c).
Conjugation	HRP

Storage & Handling

−

Storage	Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Form/Appearance	Lyophilized
Buffer/Preservatives	Preservative: 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Concentration	2.0 mg/mL
Disclaimer	For research use only

Alternative Names

−

rabbit Anti-Human IgG Fab2 Antibody peroxidase Conjugated, Rabbit Anti-Human IgG F(ab')2 Antibody peroxidase Conjugated, Rabbit Anti-Human IgG Fab2 Fragment Antibody HRP Conjugated

Human IgG F(ab')2 Peroxidase Antibody [orb346243]

Conjugation:	HRP
Purity:	This product was prepared from normal serum by delipidation, salt fractionation, ion exchange chromatography followed by pepsin digestion and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti- Human IgG, anti-Human IgG F(ab’)2 and anti-Human Serum. No reaction was observed against anti-Human IgG F(c) or anti-Pepsin.
Source:	Human

Sizes Available: 1 mg

Human IgG F(ab')2 Antibody Peroxidase Conjugated [orb347224]
Applications:
ELISA, IHC, WB
Reactivity:
Human
Species/Host:
Goat
Clonality:
Polyclonal
Conjugation:
HRP
Sizes Available: 2 mg

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at support@biorbyt.com.

Documents Download

Datasheet

Product Information

Download

Request a Document

Protocol Information

Western Blot (IB, immunoblot)

View Protocol

IHC

Immunohistochemistry

View Protocol

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

View Protocol

Find More Protocols