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Recombinant Human IL-2 Protein
SKU: orb3002442
Description
Images & Validation
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Key Properties
−| Expression System | Human Cells |
|---|---|
| Biological Activity | BLI: Loaded Human IL-2RA-Fc(Cat#orb2994463) on Protein A Biosensor, can bind Human IL-2-His(Cat#orb3002442) with an affinity constant of 3.94 nM as determined in BLI assay. Loaded Human IL-2RB-Fc(Cat#orb2994461) on Protein A Biosensor, can bind Human IL-2-His(Cat#orb3002442) with an affinity constant of 0.17 uM as determined in BLI assay.. Cell Based Assay: Measured in a cell proliferation assay using CTLL-2 mouse cytotoxic T cells The specific activity of Recombinant Human IL-2 is ≥1×107 IU/mg. |
| Tag | C-6His |
| Molecular Weight | Predicted: 16.4 KDa. Observed: 14-18 KDa, reducing conditions |
| Expression Region | Ala21-Thr153 |
| Purity | SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. (QC verified) |
| Endotoxins | < 0.01 EU/µg as determined by LAL test. |
Storage & Handling
−| Storage | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | 0.2 μm filtered solution of 10mM Sodium Citrate, 4% Mannitol, pH 4.0 |
| Reconstitution | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Interleukin-2; IL-2; T-Cell Growth Factor; TCGF; Aldesleukin; IL2
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Recombinant Human IL-2 Protein (orb3002442)
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