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Recombinant Human KGF Protein
SKU: orb3002467
Description
Images & Validation
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Key Properties
−| Expression System | E.coli |
|---|---|
| Biological Activity | ELISA: Immobilized Human KGF(Cat. No.:orb3002467) at 5 μg/mL (100 μL/well) can bind Human FGFR2 beta IIIb (C-Fc)(Cat. No.:orb2993044). The EC50 of Human FGFR2 beta IIIb (C-Fc)(Cat. No.:orb2993044) is 83 ng/mL.（Regularly tested）. Cell Based Assay: Human breast cancer organoids were cultured with Wnt3a (Cat#orb3002512), Noggin (Cat#orb3002494) , R-spondin 3 (Cat#orb2993001) , KGF(Cat#orb3002467), FGF-10 (Cat#orb3002478), EGF (Cat#orb3002493) and NRG1-beta 1 (Cat#753). DAPI (blue), Ki67(red) and ER(green). |
| Tag | Tag free |
| Molecular Weight | Predicted: 18.9 KDa. Observed: 17 KDa, reducing conditions |
| Expression Region | Cys32-Thr194 |
| Purity | SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. |
| Endotoxins | < 0.01 EU/µg as determined by LAL test. |
Storage & Handling
−| Storage | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | 0.2 μm filtered solution of 20mM Tris，1mM EDTA，5% Trehalose, 0.02% Tween 80, pH 8.0. |
| Reconstitution | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Fibroblast growth factor 7; FGF-7; Heparin-binding growth factor 7; HBGF-7; Keratinocyte growth factor; FGF7; KGF
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Recombinant Human KGF Protein (orb3002467)
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