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Description
Research Area
Cancer Biology
Images & Validation
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| Application Notes |
|---|
Key Properties
−| Source | Mammalian cell |
|---|---|
| Biological Origin | Homo sapiens (Human) |
| Biological Activity | Measured by its binding ability in a functional ELISA. Immobilized Human MAGEA4 at 2 μg/Ml can bind anti-MAGEA4 recombinant antibody, the EC50 is 30.33-43.10 ng/mL. |
| Tag | C-terminal 10xHis-tagged |
| Expression Region | 1-317aa |
| Protein Length | Full Length |
| Endotoxins | Less than 1.0 EU/μg as determined by LAL method. |
| MW | 36.7 kDa |
| Purity | Greater than 90% as determined by SDS-PAGE. |
| Protein Sequence | MSSEQKSQHCKPEEGVEAQEEALGLVGAQAPTTEEQEAAVSSSSPLVPGTLEEVPAAESAGPPQSPQGASALPTTISFTCWRQPNEGSSSQEEEGPSTSPDAESLFREALSNKVDELAHFLLRKYRAKELVTKAEMLERVIKNYKRCFPVIFGKASESLKMIFGIDVKEVDPASNTYTLVTCLGLSYDGLLGNNQIFPKTGLLIIVLGTIAMEGDSASEEEIWEELGVMGVYDGREHTVYGEPRKLLTQDWVQENYLEYRQVPGSNPARYEFLWGPRALAETSYVKVLEHVVRVNARVRIAYPSLREAALLEEEEGV |
Storage & Handling
−| Storage | The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃. |
|---|---|
| Form/Appearance | Lyophilized powder |
| Buffer/Preservatives | Lyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0 |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−(Cancer/testis antigen 1.4) (CT1.4) (MAGE-4 antigen) (MAGE-41 antigen) (MAGE-X2 antigen)
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Human MAGEA4 Protein, His Tag [orb1291062]
Unconjugated
The purity of the protein is greater than 85% as determined by SDS-PAGE and Coomassie blue staining.
The protein has a predicted molecular mass of 35.7 kDa after removal of the signal peptide. The apparent molecular mass of MAGEA4-His is approximately 35-55 kDa due to glycosylation.
Mammalian
100 μg, 10 μg, 50 μg - Item 1 of 1
- Item 1 of 1
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(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
Measured by its binding ability in a functional ELISA. Immobilized Human MAGEA4 at 2 μg/ml can bind anti-MAGEA4 recombinant antibody, the EC50 is 30.33-43.10 ng/mL.
Quick Database Links
UniProt
UniProt Details
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Protocol Information
Protein Handling and Storage Guide
Protein Handling Guide
Human MAGEA4 Protein (orb1477848)
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