Human MBD4 protein

SKU: orb753693

Description

Recombinant human MBD4 protein

Research Area

Cancer, DNA Damage, Epigenetics

Images & Validation

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Tested Applications	ELISA, WB
Application Notes	Centrifuge the vial at 10,000 rpm for 1 minute, reconstitute at 200 μg/ml in sterile distilled water by gentle pipetting 2-3 times, don’t vortex

Key Properties

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Source	E.Coli
Reactivity	Human
Tag	N-terminal His Tag or N-terminal His-IF2DI Tag, determined during production process
Molecular Weight	25.5 kDa
Expression Region	68-300
Purity	Greater than 95% as determined by SDS-PAGE

Storage & Handling

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Storage	-20°C for 12 months as lyophilized;2-8°C for 1 month under sterile conditions after reconstitution
Buffer/Preservatives	Lyophilized from a 0.2 μm filtered solution in 10 mM Hepes, 500 mM NaCl with 5% trehalose, pH 7.4
Disclaimer	For research use only

Alternative Names

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3 N(4) ethenocytosine glycosylase, G/5 fluorouracil mismatch glycosylase with biphasic kinetics, G/T mismatch glycosylase, G/U mismatch glycosylase, MBD 4, MBD4, MBD4_HUMAN, MED 1, MED1, Methyl CpG binding domain protein 4, Methyl CpG binding endonuclease 1, Methyl CpG binding protein MBD4, Methyl-CpG-binding domain protein 4, Methyl-CpG-binding endonuclease 1, Methyl-CpG-binding protein MBD4

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Quick Database Links

UniProt

O95243

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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