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Description
Images & Validation
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Key Properties
−| Expression System | Human Cells |
|---|---|
| Biological Activity | Cell Based Assay: Measured by its ability to inhibit BMP-4 induced alkaline phosphatase production by ATDC5 mouse chondrogenic cells. The ED50 for this effect is 10-100ng/mL in the presence of 200 ng/mL of Recombinant Human BMP‑4(#orb3002515).（QC verified） Human breast cancer organoids were cultured with Wnt3a(Cat#orb3002512),Noggin (Cat#orb3002494),R-spondin 3 (Cat#orb2993001),KGF(Cat#orb3002467), FGF-10(Cat#orb3002478),EGF (Cat#orb3002493)and NRG1-beta 1(Cat#753). DAPI (blue), Ki67(red) and ER(green). Human intestinal organoids (ipsc source) were cultured with Activin A(Cat#orb3002480) , BMP-4(Cat#orb3002515) , EGF (Cat#orb3002493) , Wnt3a (Cat#orb3002512), Noggin (Cat#orb3002494), R-spondin 1 (Cat#orb3002497) and FGF-4 (Cat#orb3002445) . The organoids showed good morphology. |
| Tag | Tag free |
| Expression Region | Gln28-Cys232 |
| Endotoxins | < 0.01 EU/µg as determined by LAL test. |
| MW | Predicted: 23 KDa. Observed: 25-33 KDa, reducing conditions |
| Purity | SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.. SEC-HPLC: Greater than 95% as determined by SEC-HPLC. (QC verified) |
Storage & Handling
−| Storage | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | 0.2 μm filtered solution of 20mM PB, 500mM NaCl, 2mM EDTA, pH 7.4. |
| Reconstitution | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Noggin; NOG
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Protein Handling Guide
Recombinant Human Noggin Protein (orb3002494)
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