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Human Siglec-10 Protein, Fc Tag

SKU: orb1291809

Description

Human Siglec-10, Fc (L118A, G120A, E201A) Tag is expressed from human 293 cells (HEK293). It contains AA Met 17 - Thr 546 (Accession # Q96LC7-1).

Research Area

Disease Biomarkers

Images & Validation

Application Notes
This protein carries a human IgG1 Fc (L118A, G120A, E201A) tag at the C-terminus. The protein has a calculated MW of 84.6 kDa. As the result of the mutations for Fc-Tag, the protein migrates as 90-110 kDa (Siglect-10 with Fc-Tag), 60-67 kDa (Siglect-10) and 30-32 kDa (Fc fragment) under reducing (R) condition (SDS-PAGE) due to glycosylation. Mutations (L118A, G120A, E201A /EU number: L235A/G237A/E318A) in human immunoglobulin G1 (hIgG1) Fc strongly reduce binding of the Fc mutant to cell expressed FcγRs, resulting in an almost 4-fold reduction in ADCC compared to that of wild type human IgG1.

Key Properties

Expression SystemHEK293
TargetSiglec-10
ReactivityHuman
TagC-Fc(A3)
Molecular Weight84.6 kDa
Expression RegionMet 17 - Thr 546
Purity85%
Endotoxins1.0 EU per μg
ConjugationUnconjugated

Storage & Handling

Storage-20°C
Form/AppearancePowder
Buffer/PreservativesPBS, pH 7.4
Expiration Date6 months from date of receipt.
DisclaimerFor research use only

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UniProt Details

No UniProt data available

NCBI Reference Sequences

Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product
RefSeqQ96LC7

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Protocol Information

Human Siglec-10 Protein, Fc Tag (orb1291809)

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100 μg
$ 780.00
1 mg
$ 4,450.00
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