Human TNF Alpha-Induced Protein 8-like Protein 2 (TIPE2) ELISA Kit

SKU: orb782614

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Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to TIPE2(TNF alpha-induced protein 8-like protein 2).

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Tested Applications

ELISA

Application Notes

standard: 1000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human TIPE2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human TIPE2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human TIPE2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human TIPE2 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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Reactivity	Human
Sample Types	serum, plasma, tissue homogenates and other biological fluids
Assay Type	Sandwich
Assay Time	3.5h
Range	15.63-1000 pg/mL
Sensitivity	5.7 pg/mL
Concentration	1000 pg/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Disclaimer	For research use only

Alternative Names

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TIPE2 ELISA Kit, TNF alpha-induced protein 8-like protein 2 ELISA Kit, TNFAIP8-like protein 2 ELISA Kit, TNFAIP8L2 ELISA Kit, TP8L2_HUMAN ELISA Kit, Tumor necrosis factor alpha induced protein 8 like 2 ELISA Kit, Tumor necrosis factor alpha-induced protein 8-like protein 2 ELISA Kit

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Human TNF Alpha-Induced Protein 8-like Protein 2 (TIPE2) ELISA Kit

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UniProt

Q6P589

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No UniProt data available

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Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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