Human Tryptophanyl tRNA Synthetase (WARS) ELISA Kit

SKU: orb779034

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Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Tryptophanyl tRNA Synthetase(WARS).

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Tested Applications

ELISA

Application Notes

standard: 100 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human WARS. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human WARS. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human WARS, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human WARS in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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Reactivity	Human
Sample Types	tissue homogenates, cell lysates and other biological fluids
Assay Type	Sandwich
Assay Time	3.5h
Range	1.57-100 ng/mL
Sensitivity	0.58 ng/mL
Concentration	100 ng/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Disclaimer	For research use only

Alternative Names

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GAMMA-2 ELISA Kit, IFI53 ELISA Kit, IFP53 ELISA Kit, WRS ELISA Kit, TrpRS ELISA Kit, hWRS ELISA Kit, Tryptophan tRNA Ligase 1,Cytoplasmic ELISA Kit, Interferon-induced protein 53 ELISA Kit, Tryptophanyl-tRNA synthetase ELISA Kit

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Human Tryptophanyl tRNA Synthetase (WARS) ELISA Kit

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UniProt

P23381

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No UniProt data available

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Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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