Iba1 Mouse Monoclonal Antibody

SKU: orb1294250

Description

Mouse Monoclonal antibody to Iba1

Research Area

Neuroscience

Images & Validation

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Item 1 of 3

Tested Applications	IF, IHC, WB
Dilution Range	Western Blot 1:500 1:1000, Immunofluorescence 1:100-1:200, Immunohistochemistry (Paraffin) 1:50-1:100
Reactivity	Human, Mouse, Rat

Key Properties

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Antibody Type	Primary Antibody
Host	Mouse
Clonality	Monoclonal
Isotype	Mouse IgG2a
Target	Iba1
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative
Disclaimer	For research use only

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Iba1 antibody at 1/500 dilution Lysates at 50 µg per lane. Nitrocellulose membrane was then blocked for an hour before being incubated with orb1294250 overnight at 4°C.

Iba1 monoclonal antibody orb1294250. Immunoperoxidase staining of formalin fixed, paraffin-embedded mouse brain tissue.

Immunohistochemical of frozen sections. Sample: mouse cerebellum. Green: Iba1 antibody (orb1294250): 1-200 DAPI (blue) was used as the nuclear counter stain.

Quick Database Links

Gene Symbol

Iba1

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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Immunofluorescence

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