IBMX

SKU: orb1307659

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Description

IBMX is a non-selective phosphodiesterase inhibitor that targets PDE3, PDE4, and PDE5, thereby elevating intracellular cAMP levels. It is widely used in research to study cAMP-mediated signaling pathways in cellular and tissue models, including investigations into smooth muscle relaxation, inflammation, and cardiac function.

Research Area

Cell Biology, Immunology & Inflammation, Neuroscience, Stem Cell & Developmental Biology

Images & Validation

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Key Properties

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CAS Number	28822-58-4
MW	222.3
Purity	>98%
Formula	C₁₀H₁₄N₄O₂
SMILES	CC(C)Cn1c2[nH]cnc2c(=O)n(C)c1=O
Target	PDE (Phosphodiesterase)
Solubility	Soluble in DMSO (up to 15 mg/ml) or in Ethanol (up to 10 mg/ml).

Bioactivity

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Target IC50	PDE3:6.5 μM\|THIK-1:120 μM\|PDE4:26.3 μM\|PDE5:31.7 μM
In Vivo	METHODS: To test the metabolic effects on mice, IBMX (1 mg/kg) was injected subcutaneously into mice twice daily for seven days. RESULTS: IBMX significantly increased blood glucose levels in mice (blood glucose, mg/dl, control=141, IBMX=210). METHODS: To test the metabolic effects on hyperglycemic mice, glucose (0.5 g/kg) and IBMX (1 mg/kg) were injected i.v. in femoral veins into Wistar rats. RESULTS: In hyperglycemic rats, IBMX lowered blood glucose, IBMX did not change plasma insulin levels, and IBMX decreased hepatic glycogen stores. Read more...
In Vitro	METHODS: CCDs isolated from HK-fed rats were pretreated with IBMX (100 μM) for 20 min and examined the effect of ANG II or cGMP on channel activity. RESULTS: IBMX activated ROMK channels and prevented further channel activation by ANG II. METHODS: Primary cultures of guinea pig TSMC were assayed for the effect of KMUP-1 on cAMP and cGMP levels in the presence of IBMX (100 μM). RESULTS: IBMX and KMUP-1 significantly increased cAMP and cGMP levels. The effect of KMUP-1 alone on cAMP and cGMP levels was not significantly different from that in the presence of IBMX. METHODS: Mammalian cell CHO was treated with IBMX (10-1000 μM) and whole cell currents were measured using the membrane clamp technique. RESULTS: A steady-state dose-response curve for the effect of IBMX on THIK-1 currents could be fitted with a Hill coefficient of 1 and an IC50 of 120 μM. Read more...
Cell Research	Intracellular cyclic GMP and cyclic AMP concentrations in guinea-pig TSMCs were assayed as previously described. In brief, cells were grown in 24-well plates 10^5 cells per well. At confluence, monolayer cells were washed with phosphate buffer solution (PBS) and then incubated with KMUP-1 (0.1–100 μM) in the presence of 100 μM IBMX for 20 min. Incubation was terminated by the addition of 10% trichloroacetic acid (TCA). Cell suspensions were sonicated and then centrifuged at 2500 × g for 15 min at 4°C. To remove TCA, the supernatants were extracted three times with 5 volumes of water-saturated diethyl ether. Then, the supernatants were lyophilized and the cyclic GMP or AMP of each sample was determined by using commercially available radioimmunoassay kits . Read more...
Animal Research	Male mice (25-35 g), obtained from the animal house of Faculty of Medicine, were kept in controlled environmental conditions (temperature: 23±2 oC; light-dark cycle: 7 a.m. to 7 p.m.) and were divided randomly into groups of seven. All test compounds were dissolved in DMSO and diluted to desired concentration with less than 1% DMSO. For the experiment, the test compound (IBMX, milrinone, MCPIP, mc1, mc2, mc5 or mc6) or solvent (control) was injected subcutaneously to mice at 1 mg/kg dosage twice a day (8:00 a.m. and 8:00 p.m.) for 7 days. On day 8, animals were anesthetized with intraperitoneal injection of thiopental (80 mg/kg) and blood samples were obtained from their hearts and then the liver was dissected. Each sample was centrifuged for 5 min and its serum was separated . Read more...

Storage & Handling

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Storage	RT
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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3-Isobutyl-1-methylxanthine

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at support@biorbyt.com.

Compound Identifiers

PubChem CID

3758 ↗

Key Properties

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No computed properties available.

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IBMX (orb1307659)

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Applications:	IHC-P, WB
Reactivity:	Human
Species/Host:	Rabbit
Clonality:	Polyclonal
Conjugation:	Unconjugated

Applications:	ELISA, IF, IHC
Reactivity:	Human
Species/Host:	Rabbit
Clonality:	Polyclonal
Conjugation:	Unconjugated

Applications:	ELISA, FC, WB
Reactivity:	Human
Species/Host:	Rabbit
Clonality:	Polyclonal
Conjugation:	Unconjugated

Applications:	WB
Reactivity:	Mouse
Species/Host:	Rabbit
Clonality:	Polyclonal
Conjugation:	Unconjugated

Applications:	ELISA, IF
Reactivity:	Human
Species/Host:	Rabbit
Clonality:	Polyclonal
Conjugation:	Unconjugated

IBMX

Description

Research Area

Images & Validation

Key Properties

Bioactivity

Storage & Handling

Alternative Names

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Compound Identifiers

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