IDT307

SKU: orb1691952

Description

IDT307 is a fluorescent analog of MPP+ and a substrate for the dopamine transporter (DAT). It serves as a tool compound for the rapid screening and characterization of plasma membrane monoamine transporter (PMAT) inhibitors in both cellular and biochemical assays.

Images & Validation

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Key Properties

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CAS Number	1141-41-9
MW	340.2
Purity	97.58%
Formula	C₁₄H₁₇IN₂
SMILES	[I-].C=1C=C(C=CC1C=2C=C[N+](=CC2)C)N(C)C
Target	Others
Solubility	DMSO:50 mg/mL (146.97 mM);10% DMSO+40% PEG300+5% Tween-80+45% Saline:2.5 mg/mL (7.35 mM)

Bioactivity

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In Vitro

IDT307 is an analog of the organic cation MPP+, which is transported to the parietal membrane (cerebrospinal fluid-facing side) of choroid plexus (CP) epithelial cells. Its transport process is highly sensitive to the PMAT inhibitor quinine. In Pmat knockout mice, uptake of IDT307 by choroid plexus tissue and its intracellular accumulation are significantly reduced by approximately 70%.

Cell Research

1. For PMAT inhibitor screening 1. Cell preparation: In cell culture, select a cell line suitable for the study, such as cells expressing PMAT. 2. Add IDT307: Add IDT307 to the cell culture medium, usually at a concentration of 1-50 μM. 3. Inhibitor treatment: Add a known PMAT inhibitor or potential inhibitor to the experiment, and detect fluorescence changes after a certain period of culture. 4. Fluorescence detection: Detect the fluorescence changes of IDT307 by fluorescence microscopy or fluorescence microplate reader (usually using Ex: 485 nm, Em: 535 nm wavelength). A decrease in fluorescence intensity usually means the inhibitory effect of PMAT. 2. For DAT substrate characterization 1. Cell preparation: Perform experiments in neuronal cell lines with DAT expression. 2. Add IDT307: Dissolve IDT307 in an appropriate buffer and add it to the cell culture medium. 3. Fluorescence detection: At appropriate time points, measure the fluorescence signal by fluorescence microscopy or spectrophotometer to observe DAT-mediated IDT307 transport. III. Evaluation of dopamine transporter function 1. Cell preparation: Use cell lines with DAT expression, or extract neurons from mouse brain tissue for experiments. 2. Add IDT307: Add IDT307 to the culture medium and incubate the cells for a certain period of time. 3. Fluorescence detection: Use appropriate fluorescence detection equipment to monitor the changes in the fluorescence intensity of IDT307 and then evaluate the DAT function.

Storage & Handling

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Storage	keep away from direct sunlight \| Powder: -20°C for 3 years \| In solvent: -80°C for 1 year \| Shipping with blue ice/Shipping at ambient temperature.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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IDT 307, IDT307, IDT-307

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Compound Identifiers

PubChem CID

13227270 ↗

Key Properties

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No computed properties available.

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IDT307

Description

Images & Validation

Key Properties

Bioactivity

Storage & Handling

Alternative Names

Quality Guarantee

Compound Identifiers

Key Properties

Documents Download

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Protocol Information

IDT307 (orb1691952)

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