Recombinant IgM Heavy Chain Antibody

SKU: orb2642504

Description

This antibody recognizes a protein of 75kDa, identified as the mu heavy chain of human immunoglobulins. It does not cross-react with alpha (IgA), gamma (IgG), epsilon (IgE), or delta (IgD), heavy chains, T-cells, monocytes, granulocytes, or erythrocytes. The antibody is useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin's lymphomas. The most common feature of these malignancies is the restricted expression of a single heavy chain class. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is clonal and therefore malignant.

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Tested Applications	IHC-P
Dilution Range	The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Reactivity	Human
Application Notes	The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the recombinant Heavy Chain antibody to be titered up or down for optimal performance.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. Read more...

Key Properties

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Antibody Type	Primary Antibody
Host	Mouse
Clonality	Recombinant
Isotype	Mouse IgG1, kappa
Clone No.	rIM373
Immunogen	Recombinant protein was used as the immunogen for this recombinant IgM Heavy Chain antibody.
Purification	Protein G affinity chromatography
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	Prediluted in 1X PBS, 0.1 mg/ml rAlbumin, 0.05% sodium azide; For IHC use only
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

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IHC staining of FFPE human tonsil tissue with recombinant IgM Heavy Chain antibody (clone rIM373). HIER: boil tissue sections in pH6, 10 mM citrate buffer, for 10-20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free recombinant IgM Heavy Chain antibody (clone rIM373) as confirmation of integrity and purity.

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Protocol Information

IHC-P

Immunohistochemistry Paraffin

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