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IL17A Antibody
Description
Research Area
Images & Validation
−| Tested Applications | ELISA, WB |
|---|---|
| Reactivity | Human, Mouse |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | IL-17 antibody was raised against a 16 amino acid synthetic peptide from near the amino terminus of human IL-17A.The immunogen is located within amino acids 40 - 90 of IL-17. |
| Target | IL17A |
| Molecular Weight | Predicted: 17.5kDObserved: 20 kD (Post-modification: 1 N-linked glycosylation) |
| Purification | IL-17 Antibody is affinity chromatography purified via peptide column. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | IL-17 Antibody is supplied in PBS containing 0.02% sodium azide. |
| Concentration | 1 mg/mL |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
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−IL17A Antibody [orb1239477]
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Unconjugated
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IF, IHC-Fr, IHC-P, WB
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100 μl, 200 μl, 50 μlIL17/Il17a Rabbit Polyclonal Antibody [orb402354]
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Western Blot Validation in Human THP-1 Cell Lysate. Loading: 15 µg of lysates per lane. Antibodies: IL-17 orb1239678 (A: 1 µg/mL and B: 2 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Western Blot Validation in A-20 Cell Lysate. Loading: 15 µg of lysates per lane. Antibodies: IL-17 orb1239678 (A: 2 µg/mL and B: 4 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Recombinant Protein Test. Loading: 30 ng of human IL-17 recombinant protein per lane. Antibodies: IL-17 orb1239678, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Lane 1: 0.125 µg/mL, Lane 2: 0.25 µg/mL, Lane 3: 0.5 µg/mL.
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Protocol Information
IL17A Antibody (orb1239678)
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