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IL-17A Antibody
SKU: orb345106
Description
Images & Validation
−Item 1 of 2
| Tested Applications | ELISA, WB |
|---|---|
| Dilution Range | ELISA: 1:10,000-1:50,000, WB: 1µg/mL |
| Reactivity | Human |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG2b |
| Clone No. | 4H1524.1 |
| Immunogen | Anti-IL-17A (MOUSE) Monoclonal Antibody was produced in mouse by repeated immunizations with mature full length recombinant human IL-17A produced in E.coli followed by hybridoma development. |
| Purity | Anti-Human IL-17A (MOUSE) Monoclonal Antibody was purified from concentrated tissue culture supernate by Protein G chromatography followed by extensive dialysis against the buffer stated above. This antibody is specific for human IL-17A protein. A BLAST analysis was used to suggest cross-reactivity with IL-17A from human sources based on 100% homology with the immunizing sequence. Cross-reactivity with IL-17A from other sources has not been determined. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
|---|---|
| Form/Appearance | Liquid (sterile filtered) |
| Buffer/Preservatives | Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
| Concentration | 1.00 mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Dry Ice Shipping | Please note: This product requires shipment on dry ice. A dry ice surcharge will apply. |
| Disclaimer | For research use only |
Alternative Names
−mouse anti-Interleukin-17A antibody, mouse anti-IL-17A antibody, IL-17A, Interleukin-17A, Cytotoxic T-Lymphocyte-associated Antigen 8, CTLA8, Interleukin-17, IL17, IL-17, Interleukin17
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Western Blot of Mouse Anti-IL-17A antibody. Lane 1: human full length recombinant IL-17A protein. Lane 2: mouse full length recombinant IL-17A protein. Lane 3: rat full length recombinant IL-17A protein. Load: 20 ng/lane. Primary antibody: Anti-IL-17A antibody at 1 ug/ml for overnight at 4°C.
Western Blot results of Mouse Anti-IL-17A Antibody. Lane 1: Opal Prestained Molecular Weight Ladder. Lane 2: IL-17A. Load: 50 ng. Primary Antibody: Mouse Anti-IL-17A at 1 µg/ml overnight at 4°C. Secondary Antibody: Rabbit anti-Mouse HRP at 1:40000 for 30 min at RT. Blocking: BlockOut (p/n orb348644) for 30 min at RT.
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Protocol Information
WB
Western Blot (IB, immunoblot)
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
IL-17A Antibody (orb345106)
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