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IL-17A Antibody
SKU: orb345107
Description
Images & Validation
−Item 1 of 2
| Tested Applications | ELISA, WB |
|---|---|
| Dilution Range | ELISA: 1:10,000 - 1:50,000, WB: 1µg/mL |
| Reactivity | Human |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG2b |
| Clone No. | 4H1524.1 |
| Immunogen | Anti-IL-17A (MOUSE) Monoclonal Antibody was produced in mouse by repeated immunizations with mature full length recombinant human IL-17A produced in E.coli followed by hybridoma development. |
| Purity | Anti-Human IL-17A (MOUSE) Monoclonal Antibody was purified from concentrated tissue culture supernate by Protein G chromatography followed by extensive dialysis against the buffer stated above. This antibody is specific for human IL-17A protein. A BLAST analysis was used to suggest cross-reactivity with IL-17A from human sources based on 100% homology with the immunizing sequence. Cross-reactivity with IL-17A from other sources has not been determined. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing. |
|---|---|
| Form/Appearance | Liquid (sterile filtered) |
| Buffer/Preservatives | Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
| Concentration | 1.0mg/mL |
| Expiration Date | 12 months from date of receipt. |
| Dry Ice Shipping | Please note: This product requires shipment on dry ice. A dry ice surcharge will apply. |
| Disclaimer | For research use only |
Alternative Names
−mouse anti-Interleukin-17A antibody, mouse anti-IL-17A antibody, IL-17A, Interleukin-17A, Cytotoxic T-Lymphocyte-associated Antigen 8, CTLA8, Interleukin-17, IL17, IL-17, Interleukin17
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Western Blot of Mouse Anti-IL-17A antibody. Lane 1: human full length recombinant IL-17A protein. Lane 2: mouse full length recombinant IL-17A protein. Lane 3: rat full length recombinant IL-17A protein. Load: 20 ng/lane. Primary antibody: Anti-IL-17A antibody at 1 ug/ml for overnight at 4°C.
Western Blot results of Mouse Anti-IL-17A Antibody. Lane 1: Opal Prestained Molecular Weight Ladder. Lane 2: IL-17A. Load: 50 ng. Primary Antibody: Mouse Anti-IL-17A at 1 µg/ml overnight at 4°C. Secondary Antibody: Rabbit anti-Mouse HRP at 1:40000 for 30 min at RT. Blocking: BlockOut (p/n orb348644) for 30 min at RT.
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Protocol Information
WB
Western Blot (IB, immunoblot)
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
IL-17A Antibody (orb345107)
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