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IL-17A Antibody
SKU: orb345248
Description
Images & Validation
−Item 1 of 3
| Tested Applications | ELISA, WB |
|---|---|
| Dilution Range | ELISA: 1:1,000 - 1:5,000, WB: 1:1,000 |
| Reactivity | Mouse, Rat |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | This purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant rat IL17-A protein. |
| Purity | This purified antibody has been heated to 56°C for 30 minutes. In ELISA and other immunoreactive assays, this antibody will recognize both native and recombinant rat IL17-A in cell supernatants and certain body fluids. A control of similarly diluted normal rabbit IgG is recommended. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | Preservative: None. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
| Concentration | 1.0 mg/mL |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−rabbit anti-IL-17A Antibody, rabbit anti-Interleukin-17A Antibody, Interleukin17, IL-17, Cytotoxic T-lymphocyte-associated antigen 8 and CTLA-8
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Expression of atrial fibrillation (AF)-related pro-inflammatory cytokines at 4 days after surgery. (A) Fold mRNA expression of interleukin (IL)-6, IL-1β, transforming growth factor-β1 (TGF-β1) and IL-17A. (B) Protein expression of IL-17A detected by western blot analysis. (C) Quantitative analysis of IL-17A protein expression. *P < 0.05 and **P < 0.01 vs. Sham. #P < 0.05 and ##P < 0.01 vs. IgG2a. Sham, sham-operated rats; SP, rats with sterile pericarditis.
Western blot using Biorbyt's anti-IL-17A antibody shows detection of rat recombinant IL-17A protein (arrowhead, lane 1). Approximately 2 µg of recombinant protein was loaded onto the gel. Primary antibody was used at a 1:1000 dilution. The membrane was washed and reacted with a 1:20000 dilution of DyLight™ 649 conjugated Gt-a-Rabbit IgG. Expect ~15kDa, or 30kDa homodimer. Molecular weight estimation was made by comparison to prestained MW markers indicated at the left. Other detection systems will yield similar results.
Western blot using Biorbyt's anti-IL-17A antibody shows detection of rat recombinant IL-17A protein (lane 1). Approximately 2 µg of recombinant protein was loaded onto the gel. Primary antibody was used at a 1:1000 dilution. The membrane was washed and reacted with a 1:20000 dilution of DyLight™ 649 conjugated Gt-a-Rabbit IgG. Expect ~15kDa, or 30kDa homodimer. Molecular weight estimation was made by comparison to prestained MW markers indicated at the left. Other detection systems will yield similar results.
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WB
Western Blot (IB, immunoblot)
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
IL-17A Antibody (orb345248)
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