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Il4 Antibody (Biotin)
SKU: orb1272451
Description
Research Area
Immunology & Inflammation
Images & Validation
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| Tested Applications | ELISA, WB |
|---|---|
| Reactivity | Rat |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Immunogen | Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant rat IL-4 (rat Interleukin-4). |
| Target | Il4 |
| Purification | Anti-rat IL-4 specific antibody was purified by affinity chromatography and then biotinylated. |
| Conjugation | Biotin |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Lyophilized |
| Concentration | batch dependent |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Il4e12, Il-4, Interleukin-4, B-cell IGG differentiation factor, IL-4
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To detect Rat IL-4 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.25 – 1.0 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Anti-Rat IL-4 (orb1272452) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant Rat IL-4.
To detect Rat IL-4 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Rat IL-4 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
To detect Rat IL-4 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Rat IL-4 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
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Protocol Information
WB
Western Blot (IB, immunoblot)
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
Il4 Antibody (Biotin) (orb1272451)
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