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Integin alpha 3 Recombinant Mouse Monoclonal Antibody
SKU: orb1173048
Description
Images & Validation
−Item 1 of 3
| Tested Applications | IF, IHC-Fr, IHC-P, WB |
|---|---|
| Dilution Range | WB=1:1000-5000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 |
| Reactivity | Human |
| Predicted Reactivity | Mouse |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Recombinant |
| Isotype | IgG1, Kappa |
| Clone No. | G11E8 |
| Target | ITGA3 |
| Purification | Affinity purified by Protein G |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Quality Guarantee
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Blocking buffer: 5% NFDM/TBST, Primary ab Dilution: 1:1000, Primary ab incubation condition: 4°C overnight, Lysate: HeLa, C2C12, Protein loading quantity: 20 µg, Exposure time: 30 s, Predicted MW: 120 kDa, Observed MW: 130 kDa.
Blocking buffer: 5% NFDM/TBST, Primary ab Dilution: 1:5000, Primary ab incubation condition: 4°C overnight, Lysate: A549, Protein loading quantity: 20 µg, Exposure time: 30 s, Predicted MW: 120 kDa, Observed MW: 130 kDa.
Tissue: Human adenocarcinoma, Section type: Formalin fixed & Paraffin embedded section, Retrieval method: High temperature and high pressure, Retrieval buffer: Tris/EDTA buffer, pH9.0 Primary ab Dilution: 1:100, Primary ab incubation condition: 1 hour at room temperature, Counter stain: Hematoxylin, Comment: Color brown is the positive signal for orb1173048.
Quick Database Links
Gene Symbol
ITGA3
Documents Download
Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
IHC-Fr
Immunohistochemistry Frozen
IF
Immunofluorescence
Integin alpha 3 Recombinant Mouse Monoclonal Antibody (orb1173048)
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