Irisflorentin

Irisflorentin blocks apoptosis pathways, by exerting its effects by promoting rpn-3 expression to enhance the activity of proteasomes and down-regulating egl-1 expression. Irisflorentin has anti-inflammatory mechanism in LPS-activated RAW 264.7 macrophages, by reducing the transcriptional and translational levels of inducible nitric oxide synthase (iNOS) as well as the production of NO.(In Vitro):Irisflorentin does not affect the LPS-stimulated RAW 264.7 macrophages viability up to a concentration of 40 μM.Irisflorentin (10-40 μM) inhibits the LPS-induced production of NO in a concentration-dependent manner without affecting the iNOS activity in RAW 264.7 macrophages.Irisflorentin (10-40 μM) concentration dependently inhibits the LPS (10 ng/mL)-induced increase in iNOS mRNA expression in LPS stimulated RAW 264.7 macrophages. Irisflorentin (10-40 μM) concentration-dependently suppresses the production of TNF-α, IL-1β and IL-6 in LPS stimulated RAW 264.7 macrophages.Irisflorentin (10-40 μM) concentration dependently inhibits the LPS (10 ng/mL)-induced increase in iNOS protein levels. Irisflorentin (10-40 μM) concentration-dependently suppresses LPS-induced p38 and ERK1/2 phosphorylation in a concentration-dependent manner, but slightly affects JNK phosphorylation.