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IRS-1 (phospho Ser636) rabbit pAb Antibody
SKU: orb764217
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Description
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−Item 1 of 4
| Tested Applications | ELISA, IF, IHC, WB |
|---|---|
| Dilution Range | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications. |
| Reactivity | Human, Mouse, Rat |
Key Properties
−| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human IRS-1 around the phosphorylation site of Ser636. AA range:603-652 |
| Molecular Weight | 170 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | PBS with 0.02% sodium azide and 50% glycerol pH 7.4. |
| Concentration | 1 mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Anti-IRS1 antibody, anti-Insulin receptor substrate 1 antibody, anti-IRS-1 antibody
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Western Blot analysis of various cells using Phospho-IRS-1 (S636) Polyclonal Antibody diluted at 1:2000.
Western Blot analysis of SH-SY5Y cells using Phospho-IRS-1 (S636) Polyclonal Antibody diluted at 1:2000.
Western Blot analysis of RAW using Phospho-IRS-1 (S636) Polyclonal Antibody diluted at 1:2000.
Western blot analysis of 293T 3T3 lysis using Phospho-IRS-1 (S636) antibody. Antibody was diluted at 1:2000.
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
IF
Immunofluorescence
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
IRS-1 (phospho Ser636) rabbit pAb Antibody (orb764217)
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