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ITE

SKU: orb1299983

Description

ITE is a high-affinity endogenous aryl hydrocarbon receptor (AhR) agonist with a Ki of 3 nM. It exhibits potent immunosuppressive properties and is widely used in research to study AhR-mediated immunoregulation in both cellular and animal models of inflammation and autoimmunity.

Research Area

Immunology & Inflammation, Metabolism Research

Images & Validation

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ITE

Key Properties

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CAS Number448906-42-1
MW286.31
Purity99.14% (May vary between batches)
FormulaC14H10N2O3S
SMILESCOC(=O)c1csc(n1)C(=O)c1c[nH]c2ccccc12
TargetAhR,Aryl Hydrocarbon Receptor
SolubilityDMSO:41 mg/mL (143.2 mM)

Bioactivity

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Target IC50
AhR:3 nM (Ki)
In Vivo
ITE diminishes colitis pathology through induction of Tregs; reduces inflammatory cytokines, inflammation score, and macrophage frequency; and induces DCs resulting in amelioration of colitis. Therefore, nontoxic endogenous ITE promotes the induction of Tregs and may be useful for the treatment of IBD.
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In Vitro
ITE dose- and time-dependently inhibited proliferation of HPAECs with a maximum inhibition of 83% at 20 μM after 6 days of treatment. ITE rapidly decreased AhR protein levels, while it increased mRNA levels of cytochrome P450 (CYP), family 1, member A1 (CYP1A1) and B1 (CYP1B1), indicating activation of the AhR/CYP1A1 and AhR/CYP1B1 pathways in HPAECs. The AhR siRNA significantly suppressed AhR protein expression, whereas it did not significantly alter ITE-inhibited growth of HPAECs.
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Cell Research
Immunohistochemistry was performed to localize AhR expression in human lung tissues. The crystal violet method and MTT assay were used to determine ITE's effects on growth of HPAECs. The AhR activation in HPAECs was confirmed using Western blotting and RT-qPCR. The role of AhR in ITE-affected proliferation of HPAECs was assessed using siRNA knockdown method followed by the crystal violet method
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Animal Research
At the start of DSS induction, mice received 100 μl by intraperitoneal injection of vehicle and ITE (10 mg/kg body wt) twice a week on each Monday and Thursday until week 6 at the end point of the experiment. During a pilot study, we used several (5, 10, 20, 40, and 80 mg/kg body wt) doses of ITE and noticed that the 10-mg/kg dose was the lowest dose giving maximum protection. Therefore, Used this dose in entire study. At the experimental end point blood was collected by tail-vein bleedings and serum was obtained following centrifugation. For comparison, a similar treatment was also given to normal BL/6 mice to see the effect of ITE alone.
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Storage & Handling

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StoragePowder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

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ITE, inhibit, Inhibitor, Aryl Hydrocarbon Receptor, ArylHydrocarbonReceptor, AhR

Similar Products

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  • ITE

    ITE [orb1225027]

    >98% (HPLC)

    448906-42-1

    286.3058

    C14H10N2O3S

    1 g, 500 mg, 200 mg, 5 mg, 10 mg, 25 mg, 100 mg, 2 mg, 50 mg
Quality Guarantee

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at support@biorbyt.com.

Compound Identifiers

PubChem CID
4668801 ↗

Key Properties

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No computed properties available.

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ITE (orb1299983)

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Step 1: Enter information below

(Recommended: An additional animal making an allowance for loss during the experiment)

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% DMSO +
%+
% Tween 80 +
%

Available Sizes

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2 mg
$ 80.00
5 mg
$ 100.00
1 ml x 10 mM (in DMSO)
$ 130.00
10 mg
$ 140.00
25 mg
$ 220.00
50 mg
$ 360.00
100 mg
$ 540.00
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