Keratin 20 Antibody / Cytokeratin 20 / CK20

SKU: orb3066865

Description

This MAb recognizes an intermediate filament protein of 46kDa, identified as cytokeratin 20 (KRT20). KRT20 is abundantly expressed in goblet cells and enterocytes of the gastrointestinal tract. It is a useful marker of pancreatic and colorectal cancer. KRT20 is expressed under normal, hyperplastic and neoplastic conditions.It has been detected in adenocarcinomas of the colon, stomachand biliary tract. Breast carcinomas are generally non-reactive.

Images & Validation

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Tested Applications	WB
Dilution Range	Western blot: 2-4ug/ml
Reactivity	Human
Application Notes	Optimal dilution of the Cytokeratin 20 antibody should be determined by the researcher.

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Recombinant
Isotype	Rabbit IgG, kappa
Clone No.	KRT20/3224R
Immunogen	A recombinant partial protein from human KRT20 protein (within amino acids 196-323) was used as the immunogen for the Cytokeratin 20 antibody.
Purification	Protein A affinity
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	1 mg/ml in 1X PBS; rAlbumin free, sodium azide free
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

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Quick Database Links

UniProt

P35900

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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