XRCC5/Ku80 Antibody

SKU: orb2637182

Description

The Ku protein is localized in the nucleus and is composed of subunits referred to as Ku-70 (or p70) and Ku-86 or (p86) which is also known by the synonym Ku-80 or (p80). Ku was first described as an autoantigen to which antibodies were produced in a patient with scleroderma-polymyositis overlap syndrome, and was later found in the sera of patients with other rheumatic diseases. Ku has several functions, including cell signaling, DNA replication and transcriptional activation. Ku is involved in Pol II-directed transcription by virtue of its DNA binding activity; serving as the regulatory component of the DNA-associated protein kinase that phosphorylates Pol II and transcription factor Sp. Ku proteins also activate transcription from the U1 small nuclear RNA and the human transferrin receptor gene promoters.

Images & Validation

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Tested Applications	IHC-P, WB
Dilution Range	Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
Reactivity	Human
Application Notes	Optimal dilution of the XRCC5/Ku80 antibody should be determined by the researcher.

Key Properties

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Antibody Type	Primary Antibody
Host	Mouse
Clonality	Monoclonal
Isotype	Mouse IgG1, kappa
Clone No.	XRCC5/7315
Immunogen	A portion of amino acids 300-500 was used as the immunogen for the XRCC5/Ku80 antibody.
Purification	Protein A/G affinity
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	1 mg/ml in 1X PBS; rAlbumin free, sodium azide free
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

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Western blot testing of human 1) MOLT-4 and 2) HEK293 cell lysate using XRCC5/Ku80 antibody (clone XRCC5/7315). Predicted molecular weight: 80-86 kDa.

IHC staining of FFPE human lymph node tissue with XRCC5/Ku80 antibody (clone XRCC5/7315). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human salivary gland with XRCC5/Ku80 antibody (clone XRCC5/7315). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human colon carcinoma tissue with XRCC5/Ku80 antibody (clone XRCC5/7315). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Quick Database Links

UniProt

P13010

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC-P

Immunohistochemistry Paraffin

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