L-Ascorbic Acid 2-Phosphate Sesquimagnesium Salt Hydrate (~15% Inorganics)

SKU: orb3054034

Description

L-ascorbic acid 2-phosphate (2-Phospho-L-ascorbic acid) magnesium hydrate is a long-acting vitamin C derivative that can stimulate collagen formation and expression. L-ascorbic acid 2-phosphate magnesium hydrate can be used as a culture medium supplement for the osteogenic differentiation of human adipose stem cells (hASCs).

Images & Validation

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Key Properties

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CAS Number	1713265-25-8
Purity	>98% (HPLC)
Solubility	H2O : 5 mg/mL (ultraphonic (<60°C))

Bioactivity

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In Vitro

L-Ascorbic acid 2-phosphate (0.1-1.5 mM; 2 to 3 weeks with medium exchange every 2 to 3 days) magnesium hydrate significantly stimulates cell growth, whereas addition of l-Ascorbic acid (Asc)achieves only weak growth stimulation. A combination of Asc-2P and bFGF significantly increases cell growth, but supplementation withEGF and/or insulin does not have any additional effect. L-Ascorbic acid 2-phosphate (50 μM-250 μM) magnesium hydrate is needed for the effective osteogenic differentiation of human adipose stem cells (hASCs), and higher concentrations of AsA2-P results in increased runx2 expression and ALP activity. The highest proliferation, ALP activity and runx2 expression is achieved with 150 μM AsA2-P and 10 nM dexamethasone (Dex), and 250 μM AsA2-P and 5 nM Dex. Cell Proliferation Assay Cell line: Human corneal endothelial cells (HCECs). Concentration: 0.1 mM; 0.3 mM; 1.5 mM Incubation time: 2 to 3 weeks with medium exchange every 2 to 3 days. Result: Stimulated HCEC cells growth.

Storage & Handling

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Storage	Storage temperature: -20°C. Stability: ≥ 2 years
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

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