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LGI1 Antibody
SKU: orb150838
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Description
Images & Validation
−Item 1 of 2
| Tested Applications | ICC, IF, IHC, WB |
|---|---|
| Dilution range | WB (1:1000) |
| Reactivity | Human, Mouse, Rat |
| Application Notes |
Key Properties
−| Host | Mouse |
|---|---|
| Clonality | Monoclonal |
| Isotype | IgG2a |
| Clone No. | N283/7 (Formerly sold as S283-7) |
| Immunogen | Fusion protein amino acids 37-113 (LRRNT domain and first LRR repeat) of mouse LGI1. Rat: 100% identity (77/77 amino acids identical). Human: 98% identity (76/77 amino acids identical). ~50% identity with LGI2, LGI3 and LGI4. |
| Target | LGI1 |
| Molecular Weight | 60kDa |
| Purification | Protein G Purified |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | PBS pH 7.4, 50% glycerol, 0.1% sodium azide *Storage buffer changes when conjugated |
| Concentration | 1 mg/ml |
| Disclaimer | For research use only |
Alternative Names
−LGI1, Leucine-rich glioma-inactivated protein 1, Epitempin 1, EPT, ETL1, ADLTE, ADPEAF, ADPAEF, IB1099, OTTHUMP00000020121, OTTHUMP00000020122
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Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-LGI1 Monoclonal Antibody, Clone N283/7. Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-LGI1 Monoclonal Antibody at 1:100 for overnight at 4°C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) LGI1 Antibody (D) Composite.
Western Blot analysis of Rat Brain Membrane showing detection of ~60 kDa LGI1 protein using Mouse Anti-LGI1 Monoclonal Antibody, Clone N283/7. Load: 10 μg. Primary Antibody: Mouse Anti-LGI1 Monoclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Mouse HRP at 1:200 for 1 hour at RT. Predicted/Observed Size: ~60 kDa.
Quick Database Links
UniProt Details
− No UniProt data available
NCBI Gene Details
− No NCBI Gene data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| Protein | NP_064674.1 |
|---|
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
IF
Immunofluorescence
ICC
Immunocytochemistry
LGI1 Antibody (orb150838)
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