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MEK Kinase-1 (phospho Thr1402) rabbit pAb Antibody
SKU: orb769103
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Description
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−Item 1 of 4
| Tested Applications | ELISA, IF, IHC, WB |
|---|---|
| Dilution Range | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications. |
| Reactivity | Human, Mouse, Rat |
Key Properties
−| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human MAP3K1 around the phosphorylation site of Thr1402. AA range:1368-1417 |
| Molecular Weight | 130 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | PBS with 0.02% sodium azide and 50% glycerol pH 7.4. |
| Concentration | 1 mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Anti-MAP3K1 antibody, anti-MAPKKK1 antibody, anti-MEKK antibody, anti-MEKK1 antibody, anti-Mitogen-activated protein kinase kinase kinase 1 antibody, anti-MAPK/ERK kinase kinase 1 antibody, anti-MEK kinase 1 antibody, anti-MEKK 1 antibody
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Western Blot analysis of NIH-3T3 cells using Phospho-MEK Kinase-1 (T1402) Polyclonal Antibody diluted at 1:2000.
Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using MAP3K1 (Phospho-Thr1402) Antibody.
Immunohistochemistry analysis of paraffin-embedded human brain, using MAP3K1 (Phospho-Thr1402) Antibody. The picture on the right is blocked with the phospho peptide.
Western blot analysis of lysates from Jurkat cells, using MAP3K1 (Phospho-Thr1402) Antibody. The lane on the right is blocked with the phospho peptide.
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
IF
Immunofluorescence
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
MEK Kinase-1 (phospho Thr1402) rabbit pAb Antibody (orb769103)
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