Methylisoindigotin

SKU: orb1223499

Description

Meisoindigo(Natura-α; N-Methylisoindigotin; Dian III), a derivative of Indigo naturalis, might induce apoptosis and myeloid differentiation of acute myeloid leukemia (AML).(In Vitro):Meisoindigo (Dian III; 5-20 μM; for 24 hours) inhibits growth of the AML cell lines.Meisoindigo (10 μM; for 24 hours) induces apoptosis of acute myeloid leukemia.Meisoindigo (5-10 μM; for 24 hours) causes cell-cycle arrest.Meisoindigo (5-10 μM; for 24 hours) increases the cleaved caspase-3 and pro-apoptotic Bak, and decreases Bcl-2 and Bcl-xL levels in HL-60 cells.Meisoindigo (10, 30, 50, 100, 150 μM; 24 hours) interdicts LPS-induced (1 μg/mL) NLRP3 inflammasome activation and M1/M2 polarization through down-regulation of TLR4 pathways after OGD/R in HT-22 and BV2 cells.(In Vivo):Meisoindigo (Dian III; 50-150 mg/kg; IP; daily; for 14 days) has anti-leukemic activity in vivo.Meisoindigo (2, 4, 8, 12 mg/kg; IP; before MCAO and 2 h after reperfusion) significantly reduces infarct volume, ameliorates neurological deficits 3 days after middle cerebral artery occlusion (MCAO) in Wild-type C57BL/6J mice (25-30 g). Meisoindigo reduces edema and lowers AQP4 expression in the brain.

Images & Validation

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Key Properties

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CAS Number	97207-47-1
MW	276.29
Purity	>98% (HPLC)
Formula	C₁₇H₁₂N₂O₂
SMILES	CN(C1=CC=CC=C1/C2=C3C(NC4=C\3C=CC=C4)=O)C2=O
Target	Apoptosis
Solubility	DMSO: 10 mM

Bioactivity

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In Vivo

Meisoindigo (Dian III; 50-150 mg/kg; IP; daily; for 14 days) has anti-leukemic activity In vivo. Meisoindigo (2, 4, 8, 12 mg/kg; IP; before MCAO and 2 h after reperfusion) significantly reduces infarct volume, ameliorates neurological deficits 3 days after middle cerebral artery occlusion (MCAO) in Wild-type C57BL/6J mice (25-30 g). Meisoindigo reduces edema and lowers AQP4 expression in the brain. Animal model: NOD/SCID mice, 6-8 weeks old, with HL-60 leukemic cells. Dosage: 50, 100, 150 mg/kg. Administration: IP; daily; for 14 days. Result: Had anti-leukemic activity In vivo.

In Vitro

Meisoindigo (Dian III; 5-20 μM; for 24 hours) inhibits growth of the AML cell lines. Meisoindigo (10 μM; for 24 hours) induces apoptosis of acute myeloid leukemia. Meisoindigo (5-10 μM; for 24 hours) causes cell-cycle arrest. Meisoindigo (5-10 μM; for 24 hours) increases the cleaved caspase-3 and pro-apoptotic Bak, and decreases Bcl-2 and Bcl-xL levels in HL-60 cells. Meisoindigo (10, 30, 50, 100, 150 μM; 24 hours) interdicts LPS-induced (1 μg/mL) NLRP3 inflammasome activation and M1/M2 polarization through down-regulation of TLR4 pathways after OGD/R in HT-22 and BV2 cells. Cell Viability Assay Cell line: HL-60, NB4, U937 leukemic cell lines. Concentration: 5, 10, 15, 20 μM. Incubation time: For 24 hours. Result: Inhibited growth of the AML cell lines in a dose- and time-dependent manner. Apoptosis Analysis Cell line: HL-60 cells. Concentration: 10 μM. Incubation time: For 24 hours. Result: Induced apoptosis of acute myeloid leukemia. Cell Cycle Analysis Cell line: HL-60 cells. Concentration: 5, 10 μM. Incubation time: For 24 hours. Result: Caused cell-cycle arrest, with more cells in sub-G1 and G0/G1 phases and fewer cells in the S phase, in a dose-dependent manner. Western blot analysis. Cell line: HL-60 cells. Concentration: 5, 10 μM. Incubation time: For 24 hours. Result: Increased the cleaved caspase-3 and pro-apoptotic Bak, and decreased Bcl-2 and Bcl-xL levels in HL-60 cells.

Storage & Handling

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Storage	Storage temperature: -20°C. Stability: ≥ 2 years
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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Methylisoindigotin

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Gene Symbol

Apoptosis

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