MOG (35-55) mouse, rat

SKU: orb1299620

Description

MOG (35-55) peptide is a highly immunogenic, brain-specific myelin component used to induce experimental autoimmune encephalomyelitis (EAE) in mice and rats. This model is essential for studying relapsing-remitting demyelination and neuroinflammation in multiple sclerosis research.

Images & Validation

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Key Properties

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CAS Number	149635-73-4
MW	2581.95
Purity	99.88%
Formula	C₁₁₈H₁₇₇N₃₅O₂₉S
SMILES	CSCCC(N)C(=O)NC(CCC(O)=O)C(=O)NC(C(C)C)C(=O)NCC(=O)NC(Cc1c[nH]c2ccccc12)C(=O)NC(Cc1ccc(O)cc1)C(=O)NC(CCCNC(N)=N)C(=O)NC(CO)C(=O)N1CCCC1C(=O)NC(Cc1ccccc1)C(=O)NC(CO)C(=O)NC(CCCNC(N)=N)C(=O)NC(C(C)C)C(=O)NC(C(C)C)C(=O)NC(Cc1c[nH]cn1)C(=O)NC(CC(C)C)C(=O)NC(Cc1ccc(O)cc1)C(=O)NC(CCCNC(N)=N)C(=O)NC(CC(N)=O)C(=O)NCC(=O)NC(CCCCN)C(O)=O
Target	Others
Solubility	H2O:25.84 mg/mL (10.01 mM);DMSO:Insoluble

Bioactivity

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In Vivo	METHODS: F Male C57B-/- mice (n = 20) were Subcutaneously injected with 200 μL of 200 μg Myelin Oligodendrocyte Glycoprotein Peptide (35-55), mouse, rat (MOG (35-55)). 14 days after immunization, T cells were isolated fro the spleen and purified into CD4 and CD8 T cells using CD4 and CD8 isolation kits, and the The purity was determined by flow cytometric analysis; these cells were stimulated with Myelin Oligodendrocyte Glycoprotein Peptide (35-55), mouse, rat (MOG (35-55)) and used in proliferation assays. Enzyme-linked immunosorbent assay (ELISA) was used to detect interferon-γ (IFN-γ) and interleukin (IL)-4 secretion fro the supernatants of cultured CD4 and CD8 T cells. Results After CD8/CD4 enrichment, CD8CD3 and CD4CD3 cells accounted for 86% and 94% o the total CD3 cells, respectively CD8 T cells secreted less IFN-γ and IL-4 than CD4 T cells. Read more...
In Vitro	METHODS: Dendritic cells (DCs) were loaded with MOG35-55 peptide at a concentration of 10 μg/ml. Two hours later, lipopolysaccharide (LPS, Sigma-Aldrich) was added to the cultures at a concentration of 0.25 μg/ml. Two days later (day 8 of culture), DCs were phenotyped using flow cytometry. Results In cultures of DCs loaded with Myelin Oligodendrocyte Glycoprotein Peptide (35-55), mouse, rat (MOG (35-55)) the cytokines secreted were IL-6, IL-10, IFN-γ, TNF-α, and TGF-β, with TGF-β secreted a the highest concentration IL-4 and IL-17 were not detected in any of the cultures. Read more...
Animal Research	F Male C57B-/- mice (n = 20) were induced by myelin oligodendrocyte glycoprotein (MOG)35-55 peptide.?At 14 days after immunization, T cells were isolated fro the spleen and purified as CD4 and CD8 T cells by using CD4 and CD8 isolation kits, and the The purity was determined by flow cytometric analysis. These cells were stimulated by MOG35-55 peptide and applied to proliferation assays the interferon-gamma (IFN-γ) and interleukin (IL)-4 secretion of supernatant of cultured CD4 and CD8 T cells were measured by enzyme-linked immunosorbent assays (ELISA). For adoptive transfer, recipient mice were injected with MOG35-55-specific CD8 or CD4 T cells.?EAE clinical course was measured by EAE score at 0-5 scale and spinal cord was examined by staining with hematoxylin and eosin and Luxol fast blue stainin. Read more...

Storage & Handling

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Storage	keep away from moisture,store at low temperature \| Powder: -20°C for 3 years \| In solvent: -80°C for 1 year \| Shipping with blue ice/Shipping at ambient temperature.
Disclaimer	For research use only

Alternative Names

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plaque, Myelin Oligodendrocyte Glycoprotein Peptide (35 55), mouse, rat, Myelin Oligodendrocyte Glycoprotein Peptide (3555), mouse, rat, Myelin Oligodendrocyte Glycoprotein Peptide (35-55), mouse, rat, MOG, MOG (35-55), autoimmunity, autoantigenic, demyelinating

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Compound Identifiers

PubChem CID

17838836 ↗

Key Properties

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No computed properties available.

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