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Mouse EGF
SKU: orb3002397
Description
Images & Validation
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Key Properties
−| Expression System | E.coli |
|---|---|
| Biological Activity | Cell Based Assay: Measured by its ability to inhibits the proliferation of human epithelial A431 cells.The ED50 for this effect is 2-20 ng/ml (QC verified). Human endometrial epithelial organoids were cultured with EGF (Cat#orb3002397) and R-spondin 1 (Cat#orb3002497). The organoids showed good morphology. Data were kindly provided by Novoprotein's customer. Thanks for her contribution. |
| Tag | C-6His |
| Molecular Weight | Predicted: 7.2 KDa. Observed: 9-14 KDa, reducing conditions |
| Expression Region | Asn977-Arg1029 |
| Purity | SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. |
| Endotoxins | < 1 EU/µg as determined by LAL test. |
Storage & Handling
−| Storage | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
|---|---|
| Form/Appearance | Lyophilized from a 0.2 μm filtered solution of 20mM Tris-HCl, 150mM NaCl, pH 8.0. |
| Reconstitution | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| Disclaimer | For research use only |
Alternative Names
−Pro-epidermal growth factor; Epidermal growth factor; EGF
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Mouse EGF (orb3002397)
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