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Mouse IgG F(ab')2 Antibody Peroxidase Conjugated
SKU: orb347429
Description
Images & Validation
−Item 1 of 1
| Tested Applications | ELISA, IHC, WB |
|---|---|
| Dilution range | ELISA: 1:10,000 - 1:50,000, IHC: 1:500 - 1:2,500, WB: 1:1,000 - 1:5,000 |
| Reactivity | Mouse |
| Application Notes |
Key Properties
−| Antibody Type | Secondary Antibody |
|---|---|
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Mouse IgG F(ab')2 fragment |
| Purity | Anti-MOUSE IgG F(ab')2 (GOAT) Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Mouse IgG, Mouse IgG F(ab’)2 and Mouse Serum. No reaction was observed against Mouse IgG F(c). |
| Conjugation | HRP |
Storage & Handling
−| Storage | Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide! |
| Concentration | 2.0 mg/mL |
| Disclaimer | For research use only |
Alternative Names
−Goat Anti-Mouse IgG F(ab')2 peroxidase Conjugated Antibody, Goat Anti-Mouse IgG Fab2 peroxidase Conjugated Antibody, Goat Anti-Mouse IgG Fab2 Fragment Antibody HRP Conjugation
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ELISA Results of Goat Anti-Mouse IgG F(ab')2 Antibody Peroxidase Conjugated tested against purified Mouse IgG F(ab')2. Each well was coated in duplicate with 1.0 µg of Mouse IgG (p/n orb2652749) [Red Line], Mouse IgG F(ab')2 (p/n orb346281) [Green Line]. The working dilution is 1:38000. The starting dilution of antibody was 5 µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using HRP Conjugate Stabilizer and TMB substrate (p/n orb348651).
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WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
Mouse IgG F(ab')2 Antibody Peroxidase Conjugated (orb347429)
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