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Mouse IgG Fc Antibody Peroxidase Conjugated
SKU: orb347428
Description
Images & Validation
−Item 1 of 1
| Tested Applications | ELISA, IHC, WB |
|---|---|
| Dilution range | ELISA: 1:10,000 - 1:50,000, IHC: 1:500 - 1:2,500, WB: 1:1,000 - 1:5,000 |
| Reactivity | Mouse |
| Application Notes |
Key Properties
−| Antibody Type | Secondary Antibody |
|---|---|
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Anti-Mouse IgG F(c) fragment was produced by repeated immunization with Mouse IgG F(c) fragment in goat. |
| Purity | This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Mouse IgG, Mouse IgG F(c) and Mouse Serum. No reaction was observed against Mouse IgG F(ab')2 fragment. |
| Conjugation | HRP |
Storage & Handling
−| Storage | Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide! |
| Concentration | 2.0 mg/mL |
| Disclaimer | For research use only |
Alternative Names
−Goat Anti-Mouse IgG F(c) peroxidase Conjugated Antibody, Goat Anti-Mouse IgG Fc Antibody peroxidase Conjugation, Goat Anti-Mouse IgG Fc Fragment Antibody HRP Conjugated
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Western blot of Peroxidase conjugated Goat Anti-Mouse IgG F(c) secondary antibody. Lane 1: Mouse IgG F(c). Lane 2: None. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: Peroxidase goat secondary antibody at 1:1000 for 60 min at RT. Blocking: orb348637 for 30 min at RT. Predicted/Observed size: 28 kDa, 31 kDa for Mouse IgG F(c). Other band(s): None.
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
Mouse IgG Fc Antibody Peroxidase Conjugated (orb347428)
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