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Recombinant Mouse IL-13 Protein
SKU: orb3002353
Description
Images & Validation
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Key Properties
−| Expression System | Human Cells |
|---|---|
| Biological Activity | ELISA: Immobilized Recombinant Mouse IL-13RA2 (C-Fc)(Cat#orb2992724) at 2μg/ml (100 μl/well) can bind Recombinant Mouse IL-13 (106AA,C-6His)(Cat#orb3002353).The ED50 of Recombinant Mouse IL-13 (106AA,C-6His)(Cat#orb3002353) is 9.42ng/ml.(Regularly tested). Cell Based Assay: Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. The ED50 for this effect is 2-13 ng/ml. (QC verified) |
| Tag | C-6His |
| Molecular Weight | Predicted: 12.7 KDa. Observed: 15-30 KDa, reducing conditions |
| Expression Region | Ser26-Phe131 |
| Purity | SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. |
| Endotoxins | < 1 EU/µg as determined by LAL test. |
Storage & Handling
−| Storage | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | 0.2 μm filtered solution of PBS, pH 7.4. |
| Reconstitution | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Interleukin-13; IL-13; T-Cell Activation Protein P600; Il13; Il-13
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Recombinant Mouse IL-13 Protein (orb3002353)
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