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Description
Research Area
Immunology & Inflammation
Images & Validation
−
Key Properties
−| Expression System | E.coli |
|---|---|
| Biological Activity | BLI: Loaded Human IL-2RA-Fc(Cat#orb2994463) on Protein A Biosensor, can bind Mouse IL-2(Cat#orb3002463) with an affinity constant of 0.17 uM as determined in BLI assay. . Cell Based Assay: Measured in a cell proliferation assay using CTLL‑2 mouse cytotoxic T cells.The specific activity of Recombinant Mouse IL-2 is ≥1×107 IU/mg. (QC verified) |
| Tag | Tag free |
| Expression Region | Ala21-Gln169 |
| Endotoxins | < 0.01 EU/µg as determined by LAL test. |
| MW | Predicted: 17.4 KDa. Observed: 17 KDa, reducing conditions |
| Purity | SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.. SEC-HPLC: Greater than 95% as determined by SEC-HPLC. (Regularly tested) |
Storage & Handling
−| Storage | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | 0.2 μm filtered solution of 20mM Sodium Citrate,0.02%Tween 80,4%Mannitol,pH 3.0. |
| Reconstitution | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−aldesleukin; interleukin 2; interleukin-2; IL-2; IL2; T-cell growth facter; T cell growth factor; TCGF
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Protein Handling and Storage Guide
Protein Handling Guide
Recombinant Mouse IL-2 Protein (orb3002463)
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