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Description
Research Area
Immunology & Inflammation
Images & Validation
−Item 1 of 3
| Tested Applications | IHC-P, WB |
|---|---|
| Dilution Range | IHC - 1:1000, WB - 1:500-1000, IHC-P - 1:100 |
| Reactivity | Human |
Key Properties
−| Host | Mouse |
|---|---|
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone No. | B629EV42X3X4 |
| Immunogen | This MS4A1/CD20 antibody is generated from mice immunized with a KLH conjugated synthetic peptide between 266-294 amino acids from the C-terminal region of human MS4A1/CD20. Antigen Region: 266-294 aa. |
| Target | MS4A1 |
| Molecular Weight | 33077 Da |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
|---|---|
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−B-lymphocyte antigen CD20, B-lymphocyte surface antigen B1, Bp35, Leukocyte surface antigen Leu-16, Membrane-spanning 4-domains subfamily A member 1, CD20, MS4A1, CD20
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Immunohistochemical analysis of paraffin-embedded Human tonsil section using Pink1. diluted at 1:1000 dilution. A undiluted biotinylated goat polyvalent antibody was used as the Secondary followed by DAB staining.
MS4A1/CD20 Antibody (C-term) western blot analysis in Ramos cell line lysates (35 μg/lane). This demonstrates the MS4A1/CD20 antibody detected the MS4A1/CD20 protein (arrow).
Staining MS4A1/CD20 in human tonsil tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/100) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary Antibody.
Quick Database Links
UniProt Details
− No UniProt data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| Protein | NP_690605.1, NP_068769.2 |
|---|
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Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
MS4A1/CD20 Antibody (C-term) (orb1939306)
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