MSH3 Human Over-expression Lysate

SKU: orb1365347

This product can be custom made

Description

MSH3 HEK293T cell transient overexpression lysate (as WB positive control)

Research Area

Epigenetics & Chromatin

Images & Validation

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Item 1 of 1

Application Notes

HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48 hours before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.

Key Properties

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Source	HEK293
Expression System	HEK293T
Target	MSH3
Tag	C-Myc/DDK
Molecular Weight	127.2 kDa

Storage & Handling

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Storage	Ship at ambient temperature. Upon receiving, store the sample at -20°C. Lysate samples can be reconstituted with SDS Sample Buffer. Avoid repeated freeze-thaw cycles after reconstitution. Lysate samples are stable for 12 months from date of receipt when stored at -20°C.
Form/Appearance	Components: 1 vial of 20 ug lyophilized gene-specific transient over-expression cell lysate
Expiration Date	6 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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DUP; FAP4; MRP1

Quality Guarantee

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Western blot validation of Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with transfection reagent MegaTran 2.0) using MSH3 Human Over-expression Lysate

Quick Database Links

Gene Symbol

MSH3

UniProt

P20585

RefSeq (mRNA):NM_002439

RefSeq (Protein):NP_002430

UniProt Details

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No UniProt data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

mRNA	NM_002439
Protein	NP_002430

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