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mTOR (phospho Thr2446) rabbit pAb Antibody

SKU: orb764311

Description

Rabbit polyclonal antibody to mTOR (phospho-Thr2446)

Images & Validation

Tested ApplicationsELISA, IHC, WB
Dilution RangeWB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000
ReactivityHuman, Mouse, Rat

Key Properties

ClonalityPolyclonal
IsotypeIgG
ImmunogenThe antiserum was produced against synthesized peptide derived from human mTOR around the phosphorylation site of Thr2446. AA range:2412-2461
Molecular Weight220
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesPBS with 0.02% sodium azide and 50% glycerol pH 7.4.
Concentration1 mg/ml
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

Anti-MTOR antibody, anti-FRAP antibody, anti-FRAP1 antibody, anti-FRAP2 antibody, anti-RAFT1 antibody, anti-RAPT1 antibody, anti-Serine/threonine-protein kinase mTOR antibody, anti-FK506-binding protein 12-rapamycin complex-associated protein 1 antibody, anti-FKBP12-rapamycin complex-associated protein antibody, anti-Mammalian ta antibody

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mTOR (phospho Thr2446) rabbit pAb Antibody

Western Blot analysis of various cells using Phospho-mTOR (T2446) Polyclonal Antibody diluted at 1:1000.

mTOR (phospho Thr2446) rabbit pAb Antibody

Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using mTOR (Phospho-Thr2446) Antibody.

mTOR (phospho Thr2446) rabbit pAb Antibody

Western blot analysis of lysates from NIH/3T3 cells treated with Insulin 0.01U/ml 15', using mTOR (Phospho-Thr2446) Antibody. The lane on the right is blocked with the phospho peptide.

mTOR (phospho Thr2446) rabbit pAb Antibody

Immunohistochemical analysis of paraffin-embedded human Breast cancer. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

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Protocol Information

WB
Western Blot (IB, immunoblot)
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IHC
Immunohistochemistry
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ELISA
Enzyme-linked Immunosorbent Assay (EIA)
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mTOR (phospho Thr2446) rabbit pAb Antibody (orb764311)

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50 μl
$ 250.00
100 μl
$ 390.00
DispatchUsually dispatched within 5-10 working days
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