Recombinant MUC1 Antibody / Rabbit Monoclonal

SKU: orb2640861

Description

Mucin-1/MUC1 protein is encoded by the MUC1 gene in humans. This gene encodes a membrane-bound protein that is a member of the mucin family. Family members are O-glycosylated proteins that play an essential role in forming protective mucous barriers on epithelial surfaces. MUC1 is a transmembrane protein normally expressed on the apical borders of secretory epithelial cells. Overexpression is often associated with colon, breast, ovarian, lung and pancreatic cancers. The dominant epitope of this MAb involves both amino acids as well as sugar moieties. Its epitope is destroyed by desialylation i.e. treatment with Neuraminidase.

Images & Validation

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Tested Applications	IHC-P
Dilution Range	Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1)
Reactivity	Human
Application Notes	Titration of the recombinant MUC1 may be required due to differences in protocols and secondary/substrate sensitivity.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Recombinant
Isotype	Rabbit IgG, kappa
Clone No.	MCN01-2R
Immunogen	Human milk fat globule membranes were used as the immunogen for this recombinant MUC1 antibody.
Purification	Protein A affinity chromatography
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	1 mg/ml in 1X PBS; rAlbumin free, sodium azide free
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

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Recombinant MUC1 Antibody / Rabbit Monoclonal

IHC testing of FFPE human breast carcinoma with recombinant MUC1 antibody (clone MCN01-2R). Required HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.

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UniProt

P15941

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Protocol Information

IHC-P

Immunohistochemistry Paraffin

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