Multi-rAb HRP-Goat Rabbit Recombinant Secondary Antibody (H+L)

SKU: orb2302687

Description

This Multi-rAb antibody targets to Rabbit IgG (H+L) in ELISA and Western blot. It is higly specific to rabbit IgG and shows minimal reactivity for mouse, rat, human, pig, and bovine IgGs.

Images & Validation

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Tested Applications	DOT, ELISA, WB
Dilution range	1:10,000-1:100,000 for ELISA, 1:10,000-1:200,000 for western blotting with ECL substrates (1:10,000-1:40,000 is suggested for most system).
Reactivity	Rabbit

Key Properties

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Antibody Type	Recombinant Antibody
Host	Goat
Clonality	Recombinant
Purity	The antibody was purified from culture media supernatant by immunoaffinity chromatography using Protein G beads.
Conjugation	HRP

Storage & Handling

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Storage	Store at -20°C. Stable for one year after shipment.
Form/Appearance	Liquid
Buffer/Preservatives	PBS with 50% glycerol, 10 mg/mL rAlbumin, 0.1% Proclin-300, pH 7.4.
Concentration	1.0 mg/mL
Disclaimer	For research use only

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Multi-rAb HRP-Goat Rabbit Recombinant Secondary Antibody (H+L)

Various lysates were subjected to SDS-PAGE followed by western blot with rabbit anti-TOM70 polyclonal antibody at dilution of 1:10000. Multi-rAb HRP-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (orb2302687) was used at 1:20000 for detection.

Various lysates were subjected to SDS-PAGE followed by western blot with rabbit anti-PSMB1 polyclonal antibody at dilution of 1:10000. Multi-rAb HRP-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (orb2302687) was used at 1:20000 for detection.

Various lysates were subjected to SDS-PAGE followed by western blot with rabbit anti-Beta Catenin polyclonal antibody at dilution of 1:50000. Multi-rAb HRP-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (orb2302687) was used at 1:20000 for detection.

Various lysates were subjected to SDS-PAGE followed by western blot with rabbit anti-TDP43 recombinant antibody at dilution of 1:20000. Multi-rAb HRP-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (orb2302687) was used at 1:20000 for detection.

PC-3 and Calyculin A treated PC-3 cell lysates were subjected to SDS-PAGE followed by western blot with rabbit anti-Phospho-EIF4B (Ser406) Recombinant antibody at dilution of 1:20000. Multi-rAb HRP-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (orb2302687), leading competitor’s non cross-adsorbed and cross-adsorbed secondary antibodies were all used at 0.05 μg/mL for detection.

HEK-293 and HepG2 cell lysates were subjected to SDS-PAGE followed by western blot with rabbit anti-ZO-1 polyclonal antibody at dilution of 1:50000. Multi-rAb HRP-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (orb2302687), leading competitor’s non cross-adsorbed and cross-adsorbed secondary antibodies were all used at 0.05 μg/mL for detection.

Various lysates were subjected to SDS-PAGE followed by western blot with Beta tubulin rabbit recombinant antibody at dilution of 1:20000. Two batches of Multi-rAb HRP-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (orb2302687) were used at 1:20000 for detection.

Rabbit total IgG, Mouse total IgG, Rat total IgG, Pig total IgG, Human total IgG, Bovine total IgG were coated at 100 ng/well. 0.125 μg/mL Multi-rAb HRP-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (orb2302687) was used for detection. The result indicates that orb2302694 is highly specific for rabbit IgG and does not react with other species tested in the experiment.

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Protocol Information

Western Blot (IB, immunoblot)

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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DOT

Dot Blot

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