NCAM Antibody / CD56

SKU: orb2637860

Description

This antibody reacts with an extracellular domain (close to transmembrane) of NCAM (Neural cell adhesion molecule), also called CD56. Three isoforms of NCAM / CD56 are produced by differential splicing of the RNA transcript from a single gene. The 135 kDa isoform is the basic molecule, which is glycosylated or sialylated to produce the mature species. NCAM antibody recognizes two proteins of the neural cell adhesion molecule, the basic molecule expressed on most neuroectodermally derived tissues and neoplasms (e.g. retinoblastoma, medulloblastomas, astrocytomas, neuroblastomas, and small cell carcinomas). It is also expressed on some mesodermally derived tumors (rhabdomyosarcoma). NCAM antibody plays an important role in the diagnosis of nodal and nasal NK/T-cell lymphomas.

Images & Validation

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Tested Applications	IHC-P
Dilution range	The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Reactivity	Human, Rat
Application Notes	The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM EDTA Buffer, pH 9, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. Read more...

Key Properties

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Antibody Type	Primary Antibody
Host	Mouse
Clonality	Monoclonal
Isotype	Mouse IgG1, kappa
Clone No.	123C3.D5
Immunogen	A membrane preparation of a small cell lung carcinoma was used as the immunogen for this CD56 / NCAM antibody.
Purification	Protein G affinity chromatography
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	1X PBS, pH 7.4
Disclaimer	For research use only

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IHC testing of human colon ganglion stained with NCAM antibody (123C3.D5)

SDS-PAGE Analysis of Purified, BSA-Free NCAM Antibody (clone 123C3.D5). Confirmation of Integrity and Purity of the Antibody.

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Protocol Information

IHC-P

Immunohistochemistry Paraffin

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