NCAM1 / CD56 Rabbit pAb Discontinued

SKU: orb373851

This product can be custom made

Description

Rabbit polyclonal antibody to NCAM1

Research Area

Epigenetics, Infectious Diseases

Images & Validation

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Item 1 of 6

Tested Applications	ELISA, IF, IHC-P, WB
Dilution Range	WB: 1:500-2000, IHC-P: 1:100-200
Reactivity	Human, Mouse, Rat

Key Properties

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Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 759-858 of human NCAM1 / CD56 (NP_851996.2).
Molecular Weight	120-220kDa/
Purification	Affinity purification
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	PBS with 0.05% proclin300,50% glycerol,pH7.3.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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anti-CD56 antibody, anti-cell adhesion molecule, neural, 1 antibody, anti-MSK 39 antibody, anti-MSK39 antibody, anti-N-CAM-1 antibody, anti-NCAM 1 antibody, anti-NCAM antibody, anti-NCAM C antibody, anti-NCAM-1 antibody, anti-NCAM1 antibody, anti-NCAM1_HUMAN antibody, anti-NCAMC antibody, anti-Neural cell adhesion molecule 1 antibody, anti-Neural cell adhesion molecule NCAM antibody, anti-OTTHUMP00000235666 antibody

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Immunofluorescence analysis of rat brain cells using NCAM1 / CD56 Rabbit pAb (orb373851) at dilution of 1:20 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of SH-SY5Y cells using NCAM1 / CD56 Rabbit pAb (orb373851) at dilution of 1:20 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Immunohistochemistry analysis of paraffin-embedded Mouse brain tissue using NCAM1 / CD56 Rabbit pAb (orb373851) at a dilution of 1:100 (40x lens). Microwave antigen retrieval was performed with 0.01 M Tris-EDTA buffer (pH 9.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Rat brain tissue using NCAM1 / CD56 Rabbit pAb (orb373851) at a dilution of 1:100 (40x lens). Microwave antigen retrieval was performed with 0.01 M Tris-EDTA buffer (pH 9.0) prior to IHC staining.

Western blot analysis of lysates from SH-SY5Y cells using NCAM1 / CD56 Rabbit pAb (orb373851) at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Western blot analysis of various lysates using NCAM1 / CD56 Rabbit pAb (orb373851) at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.