Neomycin sulfate

SKU: orb1310138

Description

Neomycin sulfate

Research Area

Infectious Disease & Virology, Metabolism Research

Images & Validation

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Key Properties

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CAS Number	1405-10-3
MW	908.87
Purity	99.64%
Formula	C₂₃H₄₆N₆O₁₃·3H₂SO₄
SMILES	OS(O)(=O)=O.OS(O)(=O)=O.OS(O)(=O)=O.NC[C@@H]1O[C@H](O[C@@H]2[C@@H](CO)O[C@@H](O[C@@H]3[C@@H](O)[C@H](N)C[C@H](N)[C@H]3O[C@H]3O[C@H](CN)[C@@H](O)[C@H](O)[C@H]3N)[C@@H]2O)[C@H](N)[C@@H](O)[C@@H]1O
Target	ribosome,Antibacterial,Antibiotic,Phospholipase
Solubility	DMSO:Insoluble;PBS:100 mg/mL (110.03 mM);H2O:460 mg/mL (506.12 mM)

Bioactivity

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Target IC50	PC-PLD:65 µM\|ATP-induced current:90 μM
In Vivo	METHODS: To assay in vivo activity, Neomycin sulfate (20 mg/mouse) and bacitracin (20 mg/mouse) were administered by gavage to C57BL/10 mice once daily for seven days. Results the combination of Neomycin and bacitracin decreased intestinal permeability and increased gene expression of ZO-1, JAM-A, and occludin in the ileum and ZO-1, claudin-3, and claudin-4 in the colon. METHODS: To deplet the intestinal microbiota of mice the antibiotics (ABX) vancomycin (0.5 g/L), ampicillin (1 g/L), Neomycin sulfate (1 g/L), and metronidazole (1 g/L) were administered to mice by drinking water for two weeks. Results: Antibiotics significantly reduce the diversity and composition o the gut microbiota. Read more...
In Vitro	METHODS: BHK-21 cells, VERO cells and FEA cells were treated with Neomycin sulfate (1000-20000 μg/ml) for 24 h. Cell viability was measured by MTT. Results the viability of BHK-21 cells was significantly reduced by 9000-20000 μg mL Neomycin treatment. 3000 μg mL Neomycin treatment significantly reduce the viability of FEA cells. 20000 μg mL Neomycin treatment did not affec the viability of VERO cells. METHODS: Mouse cochlear hair cells HEI-OC-1 were treated with Neomycin sulfate (2 mM for 24 h. Apoptosis was detected by Flow Cytometry. Results: 20 μM GM1 significantly reduced Neomycin sulfate-induced cell death in HEI-OC-1 cells. Read more...

Storage & Handling

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Storage	Powder: -20°C for 3 years \| In solvent: -80°C for 1 year \| Shipping with blue ice/Shipping at ambient temperature.
Disclaimer	For research use only

Alternative Names

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Framycin Sulfate, Framycin sulfate, Inhibitor, inhibit, Neomycin, Neomycin sulfate, Neomycin Sulfate, Neomycin sulphate, Antibiotic, angiogenin, aminoglycoside, Bacterial, 30S ribosome, synthesis, ribosomal, proliferation, protein, Phospholipase

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Compound Identifiers

PubChem CID

24978553 ↗

Key Properties

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No computed properties available.

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